I have extensive experience in RNA sequencing, having led multiple projects from sample preparation to data analysis. I am proficient in using tools like STAR, RSEM, and DESeq2 for alignment, quantification, and differential expression analysis. My background includes optimizing protocols for RNA extraction and library preparation, ensuring high-quality data output. I have also collaborated with bioinformatics teams to interpret complex datasets and derive meaningful biological insights.

In a fast-paced environment, I prioritize tasks by assessing their urgency and impact on the project's goals. I use project management tools like Gantt charts and Kanban boards to visualize and track progress. Regular team meetings and clear communication ensure everyone is aligned and aware of deadlines. I also maintain flexibility to adjust priorities as new information or challenges arise, ensuring that critical milestones are met without compromising quality.

Ensuring data integrity starts with rigorous experimental design and strict adherence to protocols. I implement multiple quality control checkpoints, including QC metrics from sequencing runs, alignment rates, and expression consistency across replicates. I also use tools like FastQC and MultiQC to monitor data quality throughout the process. Regular audits and cross-validation with independent datasets help identify and mitigate potential biases or errors.

Collaboration is key to successful RNA projects. I foster strong relationships by maintaining open lines of communication, regular updates, and clear documentation. I ensure that all team members understand their roles and responsibilities, and I facilitate joint meetings to align on project goals and timelines. By promoting a collaborative environment, I help bridge gaps between technical and clinical perspectives, leading to more comprehensive and impactful research outcomes.

In a previous project, we faced significant challenges with RNA degradation and low sequencing yields. To overcome this, I implemented a new RNA stabilization protocol and optimized the library preparation steps. I also increased the number of technical replicates to improve data reliability. Through iterative troubleshooting and collaboration with the sequencing facility, we were able to achieve high-quality data and successfully meet our project objectives.