I am proficient in a variety of DNA and RNA extraction techniques, including phenol-chloroform extraction, silica-based column purification, and magnetic bead-based methods. My experience spans both manual and automated extraction systems, ensuring high-quality nucleic acid yields suitable for downstream applications such as PCR, sequencing, and microarray analysis. I prioritize maintaining RNA integrity by working quickly and using RNase-free reagents and equipment.

Accuracy and precision are paramount in molecular pathology. I achieve this by strictly following standardized protocols, performing regular calibration and maintenance of equipment, and using appropriate controls in every assay. I also document all procedures meticulously and review my work for any discrepancies. Participation in proficiency testing and continuous education on emerging technologies further ensures my work meets the highest standards.

My experience with PCR and qPCR includes designing and optimizing assays, preparing reaction mixes, and analyzing amplification curves. I have worked with both SYBR Green and probe-based detection systems, focusing on achieving high specificity and sensitivity. I am also skilled in troubleshooting common issues like primer-dimer formation and non-specific amplification, ensuring reliable and reproducible results for diagnostic and research purposes.

Handling and storing sensitive biological samples require strict adherence to biosafety guidelines and proper labeling. I use personal protective equipment and work in designated areas to prevent contamination. Samples are stored at appropriate temperatures, with aliquots to avoid repeated freeze-thaw cycles. I also maintain detailed records of sample inventories and storage conditions to ensure traceability and integrity over time.

To stay updated, I regularly attend workshops and conferences, subscribe to relevant journals, and participate in professional networks. I also engage in online courses and webinars to learn about new techniques and technologies. Collaborating with colleagues and sharing knowledge within the field helps me integrate innovative approaches into my work, ensuring I contribute effectively to the evolving landscape of molecular pathology.